Journal: Nature Neuroscience

Article Title: Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain

doi: 10.1038/s41593-024-01769-2

Figure Lengend Snippet: a , Schematic diagrams showing the strategy to isolate CD45 + ;CD11b + immune cells from the CTX and GE of prenatal human brain from GW15–23. These CD45 + ;CD11b + cells are subjected to bulk RNA-seq and scRNA-seq, followed by bioinformatics analyses. The transcriptomic data are validated using immunohistochemistry (IHC), immunofluorescence microscopy (IF) and RNAscope-based in situ hybridization. Finally, CD45 + ;CD11b + cells are further characterized using high-dimensional flow cytometry and 3D Matrigel HUVEC assays. b , A volcano plot showing the genes enriched in CD45 + cells from GW20–23 (right) and those enriched in cells from GW14–19 (left). Adjusted P values and fold changes were calculated using DESeq2. By default in DESeq2, the P values attained by the Wald test are corrected for multiple testing using the Benjamini–Hochberg method. The genes shown were filtered to be below the adjusted P value of 0.05 and above a fold change of 1.2 between GW14–19 and GW20–23 comparisons (highlighted by the dashed lines). c , GSEA reveals GO terms enriched in CD45 + cells from GW14–19 and GW20–23. The data in b and c are from 21 independent biological samples. NES, normalized enrichment score; FDR, false discovery rate. d , Images taken from InCucyte S3 Live Imaging Device of HUVEC in Matrigel-based branching morphogenesis at 3, 6, 12, 24 and 48 h after plating. The conditions include 20,000 HUVEC alone and 20,000 HUVEC cocultured with 20,000 GW14–19 or GW20–23 CD45 + cells from prenatal human brain. e , Quantification of average and total endothelial branch lengths formed by HUVEC. Statistics use a two-tailed, unpaired Student’s t -test, and the data represent the mean ± standard error of the mean. The P values represent comparisons between HUVEC coincubated with CD45 + cells versus HUVEC only. Not significant comparisons are not shown. n indicates the number of independent biological samples used for quantification. For each biological sample, at least three technical replicates are used.

Article Snippet: From 0 to 48 h, vascular tube formations were imaged every 5–10 min using a 4× objective on Incucyte S3 Live-Cell Analysis Systems (Essen BioScience).

Techniques: RNA Sequencing Assay, Immunohistochemistry, Immunofluorescence, Microscopy, RNAscope, In Situ Hybridization, Flow Cytometry, Imaging, Two Tailed Test

Journal: Nature Neuroscience

Article Title: Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain

doi: 10.1038/s41593-024-01769-2

Figure Lengend Snippet: ( a ) Fluorescence-activated cell sorting (FACS) gating strategy to select for CD45 + ;CD11b + cells. ( b ) Isotype controls used in FACS. ( c ) Heatmap of critical differentially expressed genes in CD45 − and CD45 + cells used in bulk RNA-seq. ( d ) Volcano plot shows the genes enriched in CD45 + cells (right). Genes shown were filtered to be below adjusted p-value of 0.05 and above fold change of 1.2 between CD45 − and CD45 + cells. ( e ) Gene set enrichment analysis (GSEA) reveals gene ontology (GO) terms enriched in CD45 − and CD45 + cells. Data from panels c-e are from 3 independent biological samples of CD45 − cells, and 21 independent biological samples of CD45 + cells. ( f ) Images taken from InCucyte S3 Live Imaging Device of HUVEC and AAV-CMV-GFP transfected CD45 + cells from prenatal human brain samples at 18 and 40 hrs after plating. ( g ) Images taken from InCucyte S3 Live Imaging Device of HUVEC at 3, 6, 12, 24 and 48 hrs after plating. The conditions include 20,000 HUVEC alone, and 20,000 HUVEC co-cultured with 10,000 GW14-19 or GW20-23 CD45 + cells from prenatal human brain. ( h, i ) Quantification of average and total endothelial branch lengths formed by HUVEC that are co-cultured with CD45 + cells ( h ) or CD45 − cells ( i ). Statistics in panels h and i use two-tailed, unpaired Student’s t-test, data represent mean ± SEM. The P values represent comparisons between HUVECs co-incubated with CD45 + cells vs HUVECs only. Not significant comparisons are not shown. n indicates the number of independent biological samples used for quantification.

Article Snippet: From 0 to 48 h, vascular tube formations were imaged every 5–10 min using a 4× objective on Incucyte S3 Live-Cell Analysis Systems (Essen BioScience).

Techniques: Fluorescence, FACS, RNA Sequencing Assay, Imaging, Transfection, Cell Culture, Two Tailed Test, Incubation

Journal: Polymers

Article Title: Extrusion Foaming of Lightweight Polystyrene Composite Foams with Controllable Cellular Structure for Sound Absorption Application

doi: 10.3390/polym11010106

Figure Lengend Snippet: Dynamic thermal mechanical analyzer (DMA) results of different PS composites: ( a ) storage moduli ( E ′) of PS/lignin composites; ( b ) loss moduli ( E ″) of PS/lignin composites; ( c ) tan δ of PS/lignin composites; ( d ) storage moduli ( E ′) of PS/MWCNT composites; ( e ) loss moduli ( E ″) of PS/MWCNT composites; ( f ) tan δ of PS/MWCNT composites; ( g ) storage moduli ( E ′) of PS/mGr composites; ( h ) loss moduli ( E ″) of PS/mGr composites; ( i ) tan δ of PS/mGr composites.

Article Snippet: The dynamic thermal mechanical analysis was conducted using dynamic thermal mechanical analyzer (DMA, type Q-800, TA Instruments, New Castle, DE, USA).

Techniques: